Crystallization Optimizing of Cefradine

Crystallization Optimizing of Cefradine

Cefradine crystallization was studied under different conditions. The optimal conditions for crystallization were: dissolving temperature, 15°C; initial temperature in crystallization, 30°C; cooling temperature in crystallization, 0°C; and adding 1,2-propanediol as adjuvant agent with a volume ratio 0.2 of water solution. The yield of cefradine crystallization is about 92% under these condition...

Self-interaction chromatography as a tool for optimizing conditions for membrane protein crystallization.

The second virial coefficient, or B value, is a measurement of how well a protein interacts with itself in solution. These interactions can lead to protein crystallization or precipitation, depending on their strength, with a narrow range of B values (the 'crystallization slot') being known to promote crystallization. A convenient method of determining the B value is by self-interaction chromat...

A Comparison of Analytical Methods for the Content and Purity of Cefradine

Two HPLC methods such as cefadroxil and cefalexin methods were compared in their performance for the quantitative analysis of the content and purity of β-lactamic antibiotic, cefradine, for six bulk drug samples. Between the two methods, the cefadroxil method prescribed by the European Pharmacopoeia (EP) for the determination of impurities in cefradoxil was superior to the cefalexin method pres...

Design of Optimal Process Flowsheet for Fractional Crystallization Separation Process

A procedure is presented that synthesizes fractional crystallization separation processes to obtain pure solids from multi-component solutions. The method includes a procedure to generate a network flow model to identify alternative process designs for fractional crystallization. The main advantage of this systematic procedure with respect to other reported procedures is using non-equilibri...

Crystallization Screens Crystallization Optimization Cryo Screens Phasing